Journal: bioRxiv

Article Title: VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

doi: 10.1101/2021.08.30.458257

Figure Lengend Snippet: (a) Schematic representation of the VaxArray Polio Assay Kit microarray slide showing 16 replicate microarrays, (b) individual microarray layout showing 9 replicate spots in green for each serotype T(1), T(2), and T(3), and fiducial markers in grey, (c) general assay detection scheme showing serotype-specific capture with a monoclonal antibody and universal (pan-serotype) detection labeling.

Article Snippet: The VaxArray Polio Assay Kit (VXPL-9000, InDevR, Inc.) contains two microarray slides, with 16 replicate microarrays per slide, an optimized Protein Blocking Buffer (PBB), and Wash Buffer concentrates.

Techniques: Microarray, Labeling

Journal: bioRxiv

Article Title: VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

doi: 10.1101/2021.08.30.458257

Figure Lengend Snippet: Example fluorescence microarray images demonstrating reactivity and specificity of the VaxArray Polio Assay after the incubation of monovalent sIPV samples in (a) T(1), (b) T(2), (c) T(3), and a trivalent mixture containing T(1), T(2), and T(3) in (d).

Article Snippet: The VaxArray Polio Assay Kit (VXPL-9000, InDevR, Inc.) contains two microarray slides, with 16 replicate microarrays per slide, an optimized Protein Blocking Buffer (PBB), and Wash Buffer concentrates.

Techniques: Fluorescence, Microarray, Incubation

Journal: bioRxiv

Article Title: VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

doi: 10.1101/2021.08.30.458257

Figure Lengend Snippet: Comparison of % expected concentration between VaxArray Polio Assay and plate-based ELISA. D-Ag concentrations are grouped as shown on the x-axis, with the overall average % recovery over all concentrations investigated shown in the blue bars at right. (a) T(1), (b) T(2), and (c) T(3), with VaxArray Polio Assay measurements shown as solid black bars, and ELISA measurements shown as hashed black and white bars. Error bars represent % RSD of 3 replicate measurements over 2 separate experiments (n=6 for each method at each concentration).

Article Snippet: The VaxArray Polio Assay Kit (VXPL-9000, InDevR, Inc.) contains two microarray slides, with 16 replicate microarrays per slide, an optimized Protein Blocking Buffer (PBB), and Wash Buffer concentrates.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

doi: 10.1101/2021.08.30.458257

Figure Lengend Snippet: VaxArray Polio Assay measurements in trivalent cIPV-containing vaccines. IPOL and Pentacel (see for list of components in each; both contain a minimum 40/8/32 D-Ag units and were analyzed by VaxArray Polio Assay and compared to the same nominal concentrations of cIPV WHO international standard, with results shown as of expected concentration. T(1) results are shown as solid grey bars, T(2) is hashed black and white bars, and T(3) is solid black bars as shown.

Article Snippet: The VaxArray Polio Assay Kit (VXPL-9000, InDevR, Inc.) contains two microarray slides, with 16 replicate microarrays per slide, an optimized Protein Blocking Buffer (PBB), and Wash Buffer concentrates.

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: VaxArray Immunoassay for the Multiplexed Quantification of Poliovirus D-Antigen

doi: 10.1101/2021.08.30.458257

Figure Lengend Snippet: VaxArray Polio Assay measurements of Pediarix trivalent cIPV-containing vaccine indicating significantly improved recovery after citrate desorption. Results are shown as % of expected concentration based on comparison to the same nominal concentrations of cIPV WHO international standard, with error bars shown as the % RSD of triplicate measurements. T(1) results are shown as solid grey bars, T(2) is hashed black and white bars, and T(3) is solid black bars as shown.

Article Snippet: The VaxArray Polio Assay Kit (VXPL-9000, InDevR, Inc.) contains two microarray slides, with 16 replicate microarrays per slide, an optimized Protein Blocking Buffer (PBB), and Wash Buffer concentrates.

Techniques: Concentration Assay

Journal: Molecular Medicine

Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression

doi: 10.2119/2004-00045.Bischoff

Figure Lengend Snippet: mRNA expression of IGF2 and KDR in infantile hemangioma. (A) Using 32P-labeled cDNA probes to IGF2 and KDR, Northern blotting was performed on tissue RNA of proliferating hemangioma 33 and 32 from patients of 4 and 7 mo of age, involuting hemangioma I-12 and I-14 from 2 patients of 3 y of age, and normal infant skin. Equivalent loading of RNA was verified by staining the gel with ethidium bromide to visualize 28s and 18s rRNA. (B) The intensity of IGF2 and KDR signals were quantified, normalized to that of I-14, and plotted as relative fold increase.

Article Snippet: Immediately after resection, all the specimens were embedded in Optimal Cutting Temperature (OCT) compound and snap frozen in acetone/dry ice bath, or treated with RNA later TM solution (Ambion, Austin, TX, USA) and stored at −80 °C. cDNA Microarray Total RNA was isolated from proliferating hemangioma, involuting hemangioma, and normal human neonatal foreskin using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions.

Techniques: Expressing, Labeling, Northern Blot, Staining

Journal: Molecular Medicine

Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression

doi: 10.2119/2004-00045.Bischoff

Figure Lengend Snippet: Quantitative real-time PCR analysis of IGF2 and KDR transcripts in hemangioma compared with levels in other vascular anomalies. In panel A, normalized RNA levels for IGF2 (black bars) and KDR (hatched bars) were determined from a standard curve generated from cycle threshold (Ct) values obtained from serial dilutions of cDNA from human dermal microvascular endothelial cells (panel B). Slopes were −5.8 for KDR (+) and −3.6 for IGF2 (−).

Article Snippet: Immediately after resection, all the specimens were embedded in Optimal Cutting Temperature (OCT) compound and snap frozen in acetone/dry ice bath, or treated with RNA later TM solution (Ambion, Austin, TX, USA) and stored at −80 °C. cDNA Microarray Total RNA was isolated from proliferating hemangioma, involuting hemangioma, and normal human neonatal foreskin using RNeasy Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Generated